What You will Learn From This course

• Mastery of qPCR/NGS workflows and their complementary roles
• Understanding of bioinformatics pipelines for pathogen genomics
• Design diagnostic panels for African priority pathogens
• Interpret Ct values and NGS variant calls
• Improved diagnostic accuracy and outbreak response in local contexts
• Capacity to advocate for molecular diagnostic investments

 

Course Curriculum

Requirements

1. Background in laboratory science, biology, biotechnology, or related field
2. Basic understanding of genetics and diagnostic methods
3. Access to online learning materials
4. Stable internet connection for videos and assessments
5. Commitment to complete all modules and quizzes

Description

1. Course Description

 

This course provides biomedical professionals with in-depth training in cutting-edge molecular diagnostics technologies, focusing on real-time PCR (qPCR) and next-generation sequencing (NGS). Designed to bridge the gap between theory and practice, the course features case studies from African healthcare systems and emphasizes cost-effective, scalable solutions.

 

2. Objectives

 

By course completion, participants will:

 

• Explain qPCR principles (Ct values, amplification curves) and NGS technologies (library prep, sequencing platforms).
• Perform assay design for pathogen detection and analyze NGS data outputs.
• Implement quality control protocols for molecular diagnostics.
• Troubleshoot common challenges (inhibitors, low yields, contamination).
• Evaluate case studies on TB drug resistance and malaria surveillance.
• Develop implementation strategies addressing cost, training, and infrastructure gaps.

 

Instructor Profile

HealthEdu

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